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Protoplasts as tools for plant genome modifications
Author(s) -
Paszkowski Jerzy,
Peterhans Alex,
Schlüpmann Henriette,
Basse Christoph,
Lebel Edouard G.,
Masson Jean
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04746.x
Subject(s) - protoplast , genome , biology , mutagenesis , recombination , cloning (programming) , genetics , selection (genetic algorithm) , computational biology , gene , mutation , programming language , artificial intelligence , computer science
We present here the application of protoplast technology in the selection and recovery of rare, spontaneous plant genome alterations. Using protoplasts as a cell cloning system allowed the detection and molecular characterization of intrachromosomal recombination events between genomic repeats. The mechanism, frequencies and the induction of intrachromosomal recombination are discussed as well as its application for genome mutagenesis.