Premium
Electrostimulated regeneration of plantlets from protoplasts derived from cell suspensions of barley ( Hordeum vulgare )
Author(s) -
Mordhorst Andreas P.,
Lörz Horst
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04736.x
Subject(s) - protoplast , hordeum vulgare , callus , plating efficiency , regeneration (biology) , biology , transformation (genetics) , botany , somatic cell , suspension culture , poaceae , cell , microbiology and biotechnology , cell culture , biochemistry , gene , genetics
For transformation and somatic hybridisation of barley ( Hordeum vulgare L.), it is necessary to develop an efficient and reliable system for routne plant regeneration from protoplasts. Freshly‐isolated cell suspension‐derived protoplasts were treated with both rectangular and exponential electric pulses with the aim of increasing plating efficiency as well as to stimulate regenerative capacity. Suspensions were initiated from callus from immature embryos of barley (cv. Dissa). Increasing field strength, capicitance, or number of applied pulses resulted in a decreased protoplast viability and plating efficency. However, the regeneration of albino leaves and albino plantlets from electro‐treated protoplasts was stimulated in comparison with controls.