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Influence of 9‐β‐ l (+)‐adenosine on malate dehydrogenase activity in rice
Author(s) -
Savithiry Srinivasan,
Wert Violet,
Ries Stanley
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04691.x
Subject(s) - adenosine , malate dehydrogenase , oryza sativa , enzyme assay , biology , dehydrogenase , chemistry , biochemistry , enzyme , botany , gene
The activity of malate dehydrogenase (MDH, EC 1.1.1.37) in rice ( Oryza sativa L. cv. California M‐201) roots was increased within 25 min by a foliar application of 37 n M of 9‐β‐ l (+)‐adenosine [ l (+)‐adenosine]. A similar concentration of 9‐β‐ d (‐)‐adenosine [ d (‐)‐adenosine] did not affect MDH. Triacontanol (2.7 n M ) which elicits l (+)‐adenosine in plants also increased MDH activity in roots of rice seedlings. whereas neither octacosanol nor a mixture of equimolar concentrations of triacontanol and octacosanol had any effect on MDH activity. l (+)‐Adenosine increased MDH activity of rice seedlings grown at 10.20 and 40°C. The effect was measurable 24 h after application at 10 and 20°C, but not 6 h after treatment at 40°C. Ninety minutes after l (+)‐adenosine was applied to the foliage of plants grown at 10, 20 and 40°C, MDH activity in the roots was 40, 30 and 9% more, respectively, than in the untreated controls. The concentration of water soluble protein was also increased by l (+)‐adenosine and was positively correlated with MDH activity. When measured 90 min after application of l (+)‐adenosine at different times of day, MDH activity and dry weight were increased most when l (+)‐adenosine was applied 9 and 12 h after the lights came on in a 16‐h photoperiod. The optimum light intensity for the response of rice to l (+)‐adenosine, as measured by MDH activity, was 450 μmol m −2 s −1 .