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Photosynthetic competence of plantlets grown in vitro. An automated system for measurement of photosynthesis in vitro
Author(s) -
Dubé Sylvain L.,
Vidaver William
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04684.x
Subject(s) - photosynthesis , acclimatization , botany , photoinhibition , horticulture , air humidity , aseptic processing , photobioreactor , biology , humidity , agronomy , biomass (ecology) , food science , photosystem ii , physics , thermodynamics
An aseptic gas exchange and hydroponic system (AGEHS) has been developed in an attempt for characterization of physiological requirements for photoautotrophic growth in vitro and alleviation of the needs for ex vitro acclimatization. The AGEHS monitors and controls several parameters relevant to plant growth. Shootlets of Chrysanthemum × morifolium Ramat. cv. Envy were treated with flow of air or CO 2 ‐enriched air under controlled relative humidity, elevated photosynthetic photon flux density (PPFD) and hydroponic irrigation. After 15 days of treatment, plantlets gained more than 3 times as much dry weight as those from a conventional culture tube treatment. This study shows that it is possible to favour photoautotrophic growth when elevated PPFD, enhanced air‐exchange and hydroponic medium flow are provided concurrently. This enhancement is achievable through careful increments of light quanta, balanced with increments of humidified air flow and/or CO 2 content in air which seem to be necessary to avoid potential photoinhibition and premature water exhaustion from gelled media.