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Characterization of early events leading to the formation of chlorophyll‐proteins of PSII in Euglena gracilis
Author(s) -
Yamamoto Yasusi,
Mizobuchi Akihiro,
Hiramatsu Hiroyuki,
Shioi Yuzo
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04683.x
Subject(s) - euglena gracilis , euglena , chlorophyll , protochlorophyllide , photosystem ii , chlorophyll a , greening , thylakoid , biology , light harvesting complexes of green plants , chloroplast , photosystem i , biophysics , photosynthesis , chemistry , botany , biochemistry , gene , ecology
Development of chlorophyll‐proteins in photosystem II was studied with Euglena gracilis Z. during dark‐light transition. Upon illumination of the dark‐grown cells, protochlorophyllide was photoconverted to chlorophyll(ide) a with a low efficiency (14%). After a lag time of 1‐2 h, chlorophylls, apoproteins of antenna chlorophyll‐protein complex CP 43/47 and of light‐harvesting chlorophyll‐protein complex (LHCII) accumulated in the thylakoid membrane in a coordinated fashion. There was, however, a significant difference in the stability between the newly formed LHCII and CP 43/47 judging from non‐denaturing lithium dodecyl sulfate‐polyacrylamide gel electrophoresis. The possibility that efficiencies of incorporation and stabilization of chlorophylls in the apoproteins differ among the chlorophyll‐proteins in the early stage of greening of Euglena is discussed.