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Cell cycle and cell differentiation in lentil roots grown on a slowly rotating clinostat
Author(s) -
Legué Valérie,
DrissEcole Dominique,
Perbal Gérald
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04680.x
Subject(s) - clinostat , elongation , mitosis , cell cycle , microbiology and biotechnology , mitotic index , chemistry , root tip , biophysics , biology , botany , cell , materials science , biochemistry , metallurgy , ultimate tensile strength
Root growth was studied for seedlings of lentil ( Lens culinaris L. cv. Verte du Puy) grown for 27 h either on a slowly rotating clinostat (0.9 rev. min −1 ) of vertically (controls). Horizontal clinorotation was employed, so that the longitudinal axis of the root was parallel to the axis of the rotation. Morphological (root length and orientation) and cellular (cell proliferation and cell elongation) parameters were studied. The cell cycle was also analysed by flow cytometry. Root length deviation of the roots from the initial orientation was observed on the clinostat; this deviation could be due to spontaneous oscillation. Cell elongation of the clinostat‐rotated roots occurred closer to the tip than in the vertical roots, but the mitotic index was not modified. Clinorotation did not change the frequencies of the G1, S and G2 phases of the cell cycle. These results were compared to those obtained during the D1 mission on Spacelab, 1985. The effects of microgravity on root orientation and mitotic index were not simulated by clinorotation.