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Multiple forms of ADPglucose pyrophosphorylase of rice endosperm
Author(s) -
Nakamura Yasunori,
Kawaguchi Kentaro
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04673.x
Subject(s) - endosperm , polyclonal antibodies , oryza sativa , polyacrylamide gel electrophoresis , biochemistry , amino acid , chemistry , electrophoresis , biology , enzyme , antibody , genetics , gene
ADPglucose pyrophosphorylase from developing rice ( Oryza sativa ) endosperm was purified. The final preparation yielded 6 major protein spots as separated by two‐dimensional polyacrylamide electrophoresis. All 6 polypeptides had similar molecular weights of ca 50 kDa and cross‐reacted with polyclonal antibodies raised against two main protein bands among them. The results suggest that the rice endosperm ADPglucose pyrophorsphorylase is tetrameric and composed of multiple subunits with similar amino acid structure.