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Strategies in the purification of plant proteins
Author(s) -
Weselake Randall J.,
Jain Jinesh C.
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb04668.x
Subject(s) - affinity chromatography , chemistry , chromatography , metal ions in aqueous solution , biochemistry , computational biology , biology , metal , organic chemistry , enzyme
This review focuses on some recent developments in the purification of plant proteins. Topics discussed include chromatographic procedures using immobilized antibodies, protein subunits and metal ions. The use of affinity labeling to facilitate purification is presented along with recent uses of detergents. Some of the methodology has had limited application in plant biochemistry, and where appropriate, the authors have presented examples of results with non‐plant systems.