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Cell wall glycanases and their activity against the hemicelluloses from pine hypocotyls
Author(s) -
Acebes José Luis,
Zarra Ignacio
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb01340.x
Subject(s) - depolymerization , xyloglucan , polysaccharide , xylose , cell wall , chemistry , arabinose , hemicellulose , biochemistry , lignin , hydrolysis , organic chemistry , fermentation
Glycanases associated with the cell walls of Pinus pinaster Aiton from Northern Spain (29QT638, altitude 1000 m) were extracted with 1 M LiCl solution, and their effect on the hemicellulosic polysaccharides from the same material was studied. The extracted proteins were able to increase the amount of reducing sugars and to decrease the viscosity of the hemicellulosic polysaccharides extracted with both 4 and 24% (w/v) KOH, suggesting the presence of exo‐ and endo‐type glycanases. The extracted proteins released arabinose, galactose and xylose from both hemicellulosic polysaccharides, and caused an important depolymerization of xyloglucan. The results indicate that the changes in the hemicellulosic polysaccharides, i.e., xyloglucan depolymerization, found during cell elongation are caused by an enzymic system associated with the cell wall.