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Internode length in Pisum . A new allele at the Lh locus
Author(s) -
Swain Stephen M.,
Reid James B.
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb01320.x
Subject(s) - allele , locus (genetics) , pisum , sativum , biology , genetics , endogeny , gene , botany , biochemistry
A new allele at the Lh locus has been identified in Pisum sativum L. and named lh i . This allele results in reduced GA levels in young shoots, and a dwarf phenotype. Gas chromatography‐selected ion monitoring (GC‐SIM) with dideuterated internal standards has been used to demonstrate a quantitative relationship between the level of endogenous GA 1 and internode length using the three alleles ( Lh, lh and lh i ) at the Lh locus. These results are consistent with previous findings in peas (for alleles at the Le locus) and other species possessing a predominant early 13‐hydroxylation pathway for GA biosynthesis and support the role of GA 1 as the major native GA in peas with biological activity in its own right. However, in contrast to alleles at the Le locus, GA 20 levels are also reduced in lh and lh i plants. The lh i allele also has possible pleiotropic effects on seed abortion, leading to a reduction in seed yield compared to plants homozygous for the previously characterised Lh or lh alleles.