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Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole‐3‐acetic acid
Author(s) -
Veselov Stanislav Yu.,
Kudoyarova Guzel R.,
Egutkin Naum L.,
GyuliZade Vaid Z.,
Mustafina Aelita R.,
Kof Ella M.
Publication year - 1992
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1992.tb01316.x
Subject(s) - immunoassay , solvent , chromatography , chemistry , acetic acid , indole 3 acetic acid , distribution (mathematics) , biochemistry , biology , mathematics , immunology , auxin , antibody , gene , mathematical analysis
Based on the distribution constant of IAA, the efficiency of solvent partitioning has been improved by modifying the proportions of the solvents. IAA is recovered almost quantitatively by this method which also renders further sample reduction superfluous. Selective IAA recovery is supported by the distribution of immunoreactive materials on chromatograms. This modified scheme simplifies prepurification of samples for more reliable immunoassay.