Premium
Investigations of proliferative and senescent callus of soybean
Author(s) -
Keese Renee J.,
Rupert Earlene A.,
Carter George E.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb05093.x
Subject(s) - callus , mucilage , explant culture , botany , chloroplast , meristem , biology , plastid , chemistry , in vitro , biochemistry , shoot , gene
Different explant sources from several Giycine max (L.) Merr. cultivars and several G. soja F. J. Herm Plant Introductions were tested for calluas production. Embryo axis explants produced callus most readily; hence, they were selected for media response studies and microscopic evaluation. Two callus types, proliferative and senescing, were identified and characterized by light and scanning electron microscopy (SEM). Proliferative callus was composed of chloroplast‐containing cells interspersed with colorless meristematic areas, while senescing callus contained mostly large, vacuolate cells without chloroplasts. In SEM preparations a furry, layered, mucilaginous coating was seen on the outer surface of the senescing callus, while proliferative callus cells had uncoated, smooth or wrinkled surfaces. The mucilage contained glucose and galactose.