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Activation of the non‐electrochromic component in the 518 nm absorbance change by photosystem I
Author(s) -
Ooms Jaap J. J.,
Versluis Wilma,
Vredenberg Wim J.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02948.x
Subject(s) - p700 , electrochromism , absorbance , photochemistry , dcmu , photosystem i , photosystem ii , chemistry , photosystem , analytical chemistry (journal) , photosynthesis , electrode , biochemistry , chromatography
The flash‐induced absorbance change measured at 518 nm (P515) in intact chloroplasts consists of at least 4 kinetically different components. Here the non‐electrochromic component, either called phase d or reaction 3, is studied in some detail. The effect of DCMU, DQH 2 and DBMIB on the amplitude of reaction 3 and the turnover of cytochrome f and P700 have been monitored, suggesting an involvement of photosystem 1 in the activation of the non‐electrochromic absorbance change. This is confirmed by the parallel oscillation pattern found in P700 rereduction and the amplitude of reaction 3.