Premium
Stimulation of bicarbonate incorporation by ammonium in nonphotosynthetic cell suspension cultures of Acer pseudoplatanus
Author(s) -
Goodchild Jonathan A.,
Givan Curtis V.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02944.x
Subject(s) - ammonium , stimulation , bicarbonate , glutamine synthetase , biochemistry , pyruvate carboxylase , enzyme , ammonium bicarbonate , chemistry , hepes , acer pseudoplatanus , glutamine , biology , organic chemistry , amino acid , neuroscience , raw material
Ammonium supplied to Acer cells incubated in Tricine buffer raised net dark incorporation of 14 C‐bicarbonate by 1.5 to 3.3 fold; this stimulation was not abolished by prior inhibition of glutamine synthetase by methionine sulphoximine. With cells in phosphate buffer, ammonium gave a smaller 1.15 to 1.3 fold stimulation which was abolished when glutamine synthetase was inhibited. Ammonium had no direct effect on the activities of phosphoenolpyruvate carboxylase and pyruvate kinase assayed in partially purified extracts or on the enzymes catalysing release of label.