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Glycine oxidation in mitochondria isolated from light grown and etiolated plant tissue
Author(s) -
Lernmark Ulrika,
Henricson Dag,
Wigge Bosse,
Gardeström Per
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02915.x
Subject(s) - etiolation , glycine , pisum , hordeum vulgare , sativum , photosynthesis , biology , biochemistry , mitochondrion , hordeum , botany , poaceae , amino acid , enzyme
Mitochondria were isolated from light grown and dark grown monocotyledonous (wheat‐ Triticum aestivum and barley‐ Hordeum vulgare ) and dicotyledonous (pea‐ Pisum sativum ) plants and their capacity to oxidize glycine was measured. In all of the studied plant species the rate of mitochondrial glycine oxidation was high in light grown leaves. Glycine oxidation in mitochondria from etiolated leaves was also very substantial; the rate of glycine oxidation relative to the oxidation of other substrates was about half as compared to green tissue. In etiolated non‐photosynthetic tissues the relative glycine oxidation was only ca 20% of that measured in green leaves. The effect of light on the development of glycine oxidation capacity was studied using etiolated barley which was transferred to light for 6 to 24 h. During this time the rate of glycine oxidation as compared to the oxidation of NADH and malate increased, approaching the ratio observed in light grown leaves. It is concluded that the synthesis of proteins involved in glycine oxidation is regulated both in a light dependent and in a tissue specific manner. Monocotyledonous plants should be very useful for further studies of this aspect due to the relatively small developmental difference between etiolated and light grown leaf tissue.