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Effect of auxins on spermidine uptake into carrot protoplasts
Author(s) -
Kanchanapoom Malee,
Antogi Fabiana,
Pistocchi Rossella,
Bagni Nello
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02897.x
Subject(s) - spermidine , auxin , protoplast , daucus carota , vanadate , biochemistry , spermine , indole 3 acetic acid , biology , chemistry , botany , enzyme , gene
The effect of an auxin, indole‐3‐acetic‐acid (IAA), on spermidine uptake into protoplasts of carrot ( Daucus carota L. cv. Ingrid) was studied. In the presence of 1 m M Ca 2+ , IAA (10 −7 to 10 −4 M ) enchances [ 14 C]‐spermidine uptake into carrot protoplasts, while no stimulation occurs in the absence of Ca 2+ . The time course of the uptake with and without IAA is very rapid and reaches saturation within 1 to 2 min. Preincubation of protoplasts with IAA inhibits the spermidine uptake. La 3+ , known not to penetrate the plasmalemma, exerts the same effect as Ca 2+ , but gives lower uptake values than Ca 2+ . The application of vanadate, an ATPase inhibitor, strongly inhibits IAA‐stimulated spermidine uptake, suggesting that an energy‐dependent mechanism may be involved in this transport. Neither spermidine nor Ca 2+ alone stimulate IAA uptake. The synthetic auxin 2,4‐dichlorophenoxyacetic acid, yields the same results as IAA with regard to time course of spermidine uptake with and without preincubation while, unlike IAA, no significant effect was observed on the Ca 2+ ‐induced increase of spermidine uptake.