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Further studies on the effects of different auxins and cytokinins on flower formation in thin cell layers of Nicotiana tabacum : The effects of zeatin riboside, dihydrozeatin and dihydrozeatin riboside
Author(s) -
Heylen Christine,
Vendrig Jan C.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02470.x
Subject(s) - riboside , auxin , cytokinin , kinetin , zeatin , biology , nicotiana tabacum , botany , bud , explant culture , biochemistry , in vitro , gene
Organogenesis in thin cell layers of Nicotiana tabacum L. was studied in relation to the effects of natural and synthetic auxins in combination with various cytokinins. All cytokinins tested, benzyladenine (BA), kinetin, zeatin (Z), zeatin riboside (ZR), N 6 ‐Δ 2 ‐isopentenyl) adenine (IPA), dihydrozeatin [(diH)Z] and dihydrozeatin riboside [(diH)ZR], seem to be active in flower bud formation. In addition to the initiation of flower buds, vegetative buds or roots were also formed on the explants in the presence of BA, Z or IPA as exogenous cytokinins. Only dihydrozeatin and its riboside stimulated the initation of flower buds alone (as is known for kinetin), especially if supplemented with indole‐3‐acetic acid (IAA) as exogenous auxin. A high number of explants with flower buds was also found with high cytokinin/2,4‐D ratios. In these conditions the presence of (diH)Z yielded the higest number of flower buds per explant.