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Stability of salt tolerance at the cell level after regeneration of plants from a salt tolerant tobacco cell line
Author(s) -
Watad A. A.,
Swartzberg D.,
Bressan R. A.,
Izhar S.,
Hasegawa P. M.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02158.x
Subject(s) - nicotiana tabacum , salt (chemistry) , solanaceae , regeneration (biology) , biology , botany , cell culture , plant stem , wild type , plant cell , plant physiology , horticulture , chemistry , microbiology and biotechnology , biochemistry , mutant , genetics , gene
Plants were regenerated from both the wild type and a stable NaCI‐tolerant line of tobacco cells ( Nicotiana tabacum/gossii ). The regeneration process was much more difficult in the case of the NaCI‐tolerant line and was only successful in the absence of NaCI. These plants differed morphologically from those regenerated from the wild type cell line, exhibiting abnormally short internodes, small leaves and reduced growth. Cell suspension cultures derived from plants regenerated from the stable NaCI‐tolerant line retained a high level of tolerance to salt. The NaCI‐concentration required to reduce fresh and dry weight gain by 50% was about twice that observed in the case of the cells obtained from wild type plants. The results presented here, together with those of Watad et al. (1985), indicate that resistance to salt is operating and stable at the cellular level before and after plant regeneration. When the regenerated plants were grown in increasing levels of salt their growth response was not clearly different from that of the plants regenerated from the wild type cell line. However, the survival of plants on high concentrations of NaCI tended to be higher in the case of plants regenerated from the NaCI‐tolerant cell line.