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In vivo labelling of the proteins in the first leaf of intact oat plants ( Avena sativa )
Author(s) -
Klerk H.,
Tophof S.,
Loon L. C.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02147.x
Subject(s) - avena , methionine , labelling , incubation , polyacrylamide gel electrophoresis , biology , in vivo , chemistry , ethanol , botany , biochemistry , enzyme , amino acid , microbiology and biotechnology
In order to label the primary leaves of intact oat plants ( Avena sativa L. cv. Victory) for studying protein turnover using two‐dimensional polyacrylamide gel electrophoresis, the following methods were tested: growth of seedlings on 35 S‐sulfate‐containing Knop medium, labelling with 35 S‐methionine by vacuum infiltration of the leaf, injection into the leaf base or into the seed near the embryo, or wiping the surface of the leaf with ethanol followed by incubation in the labelled solution. A specific activity of 1.6 kBq per 20 μg of leaf protein applied was minimally necessary to obtain a well‐resolved fluorogram of the gels. This level of labelling was reached only upon the treatment with ethanol, which did not require more than 0.55 MBq of 35 S‐methionine per leaf. The method may be useful locally to apply compounds to intact plants.