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Effects of spermidine on the synthesis of α‐amylase in cotyledons of mung bean seedlings
Author(s) -
Koizuka Nobuya,
Tanaka Yoshiyuki,
Morohashi Yukio
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb02131.x
Subject(s) - spermidine , amylase , vigna , imbibition , biology , radiata , biochemistry , enzyme , germination , botany
When cotyledons of mung bean [ Vigna radiata (L.) Wilczek] were treated with spermidine (3 m M ) during the first 6 h of imbibition, the development of α‐amylase activity in cotyledons during the following 3 days was severely inhibited (75%) This inhibition was due to a slower accumulation of α‐amylase protein, which in turn resulted from an inhibition of α‐amylase synthesis. The rise in the level of α‐amylase mRNA in cotyledons was also inhibited by spermidine treatment. However, the degree of inhibition of mRNA accumulation (40%) was not so marked as that of the activity of α‐amylase synthesis (80%). These results are discussed in relation to the mode of action of spermidine on α‐amylase expression.