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The greening process in cress seedlings. I. Pigment accumulation and ultrastructure after application of 5‐aminolevulinate and complexing agents
Author(s) -
Kittsteiner Ursula,
Mostowska Agnieszka,
Rüdiger Wolfhart
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb01726.x
Subject(s) - greening , pigment , chelation , chlorophyll , chemistry , chloroplast , ultrastructure , lepidium sativum , chlorophyll a , botany , biochemistry , biophysics , biology , organic chemistry , ecology , germination , gene
A reduced rate of greening after continuous illumination was observed in dark‐grown cress seedlings ( Lepidium sativum L.) incubated with 5‐aminolevulinate (ALA) or the complexing agents 2,2′‐bipyridyl, 8‐hydroxyquinoline or 1,10‐phenanthroline. This effect cannot be explained merely by photodynamic damage caused by chlorophyll precursors which are accumulated in the dark under these conditions. Flash light experiments revealed that photoconversion of protochlorophyll(ide) to chlorophyllide was not influenced by chelator treatment. The next step in the chlorophyll pathway, the esterification of chlorophyllide, however, was inhibited. Simultaneously applicated ALA and complexing agents did not result in a synergistic reponse; on the contrary, ALA seemed to render cress plants less susceptible to the treatment with complexing agents upon subsequent irradiation. Ultrastructural studies demonstrated that grana formation in light was inhibited after pretreatment with ALA or complexing agents.