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An oxygen electrode assay to determine autoclaved‐induced toxicity in tissue culture media
Author(s) -
Hsiao KeCheng,
Bornman Chris H.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb01712.x
Subject(s) - oxygen , fructose , sucrose , cyanide , chemistry , clark electrode , germination , pollen , biochemistry , food science , electrode , botany , biology , inorganic chemistry , organic chemistry , electrolyte
An oxygen electrode was used to assay cyanide‐induced oxygen consumption of 8 sugars following autoclaving and the results correlated with the effects of the same sugars (reported by de Lange, S. Afr. J. Bol. 55, 1, 1989) on pollen germination and pollen tube growth. Of the sugars tested, fructose was the least stable, producing a degradation product that resulted in a 40‐fold higher consumption of oxygen than sucrose. The use of cyanide‐induced oxygen uptake makes it possible to control more carefully the conditions of preparation of sterile media by autoclaving.