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Cytochemical and X‐ray microanalytical studies of calcium in the extending zone, particularly in the rhizodermis of Zea mays roots
Author(s) -
Jaunin Françoise,
RouelleRossier Viviane Boutinard,
Hofer RoseMarie
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb01706.x
Subject(s) - antimonate , calcium , vacuole , endoplasmic reticulum , microanalysis , chemistry , nucleus , cytochemistry , staining , biophysics , ultrastructure , elongation , electron microscope , zea mays , transmission electron microscopy , cytoplasm , botany , nuclear chemistry , biochemistry , biology , inorganic chemistry , materials science , microbiology and biotechnology , metallurgy , antimony , organic chemistry , genetics , physics , ultimate tensile strength , optics , nanotechnology , agronomy
Calcium was detected in CaCl (10 m M )‐pretreated roots of Zea mays L. (cv. LG 11) by means of electron probe X‐ray microanalysis with energy‐dispersive spectrometry (EDS) using embedded samples, fixed by the antimonate staining procedure. A high level of calcium was found where large amounts of antimonate precipitates were observed by light or transmission electron microscopy. In the elongation zone, after 20 h in humid air following a 2 h CaCl 2 pretreatment, the level of calcium was higher in trichoblasts than in atrichoblasts. In these cells it was detected mainly in the walls and nucleus, and antimonate staining was observed in the walls. Abundant precipitates containing calcium were associated with the nucleus, vacuoles, mitochondria and endoplasmic reticulum of non‐differentiated cells, whereas they were confined to the walls of these cells just after the CaCl 2 pretreatment. The involvement of calcium in the formation of root hairs is discussed.