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Stimulation by 2,4‐dichlorophenoxyacetic acid of betacyanin accumulation in suspension cultures of Phytolacca americana
Author(s) -
Sakuta Masaaki,
Hirano Hiroshi,
Komamine Atsushi
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb01295.x
Subject(s) - tyrosine , suspension culture , biosynthesis , stimulation , biology , biochemistry , chemistry , cell culture , enzyme , genetics , neuroscience
2,4‐Dichlorophenoxyacetic acid (2,4‐D) strongly promoted betacyanin accumulation in suspension cultures of Phytolacca americana L. The betacyanin accumulation attained a maximum at 5 μ M 2,4‐D, when betacyanin content per cell reached 252% as compared to the control (2,4‐D free). 2,4‐D elevated the level of free tyrosine, which is the precursor of betacyanin. The addition of 1 m M tyrosine to the medium partially reversed the reduction of betacyanin accumulation caused by the removal of 2,4‐D. Tracer experiments using labelled tyrosine showed that 2,4‐D activated the biosynthetic pathway from tyrosine to betacyanin. These results indicate that a sufficient supply of tyrosine and the activation of biosynthesis of betacyanin from tyrosine by 2,4‐D elevate the level of betacyanin.