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Responsiveness to abscisic acid of embryos of dormant oat ( Avena sativa ) seeds. Involvement of ABA‐inducible proteins
Author(s) -
Corbineau F.,
PoljakoffMayber A.,
Côme D.
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb01273.x
Subject(s) - abscisic acid , avena , germination , imbibition , dormancy , embryo , biology , seed dormancy , storage protein , caryopsis , biochemistry , botany , chemistry , microbiology and biotechnology , gene
Abscisic acid (ABA) inhibited the germination of embryos isolated from oat ( Avena sativa L.) dormant seeds. The inhibition increased with ABA concentration. The embryos were 1 000 times more responsive to ABA at 30°C than at 10°C (30°C being the temperature at which dormancy is expressed). Exposure of embryos to 0.5 μ M ABA for less than 8 h did not affect subsequent germination in water, and did not induce synthesis of new proteins. Exposure for at least 14 h was necessary to induce subsequent inhibition of germination. Such long exposure to ABA also affected protein synthesis. The molecular weights of the main ABA‐inducible proteins were in the range between 44 and 24 kDa. Synthesis of small polypeptides, around 10 and 6 kDa was also induced. Synthesis of most of these ABA‐inducible proteins disappeared when embryos were transferred to water. The general results suggest that the presence of ABA was necessary to maintain the synthesis of ABA‐inducible proteins; on removal of ABA their synthesis ceased. No apparent correlation between these proteins and germination was revealed. However, a 26 kDa band was observed in the extract of control embryos imbibed for 24 h in water: this band was not present after 8 or 16 h imbibition and was also absent in extracts of ABA treated embryos.