Premium
Reversal by light of deleterious effects of chilling on oxygen evolution, manganese and free fatty acid content in tomato thylakoids is not accompanied by restoration of the original membrane conformation
Author(s) -
Garstka Maciej,
Kaniuga Zbigniew
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb00096.x
Subject(s) - thylakoid , oxygen evolution , photosynthesis , chemistry , activation energy , biophysics , manganese , oxygen , chloroplast , botany , biochemistry , biology , electrochemistry , organic chemistry , electrode , gene
Free fatty acids (FFA) generated in thylakoids upon chilling of tomato leaves at 0°C for a few days result in release of functionally active Mn and inactivation of O 2 evolution. Chilling does not lead to a decrease in the extrinsic 16, 23 and 33 kDa polypeptides. Upon illumination of chilled leaves both Mn content and O 2 evolution in thylakoids are restored and FFA content is reduced to the level of the control. Photoactivation of O 2 evolution in chilled leaves does not change the ratio of unsaturated/saturated FFA. Constant Arrhenius activation energy (Ea) for O 2 evolution by thylakoids isolated from control leaves was found, whereas it increased at temperatures below 8.0 and 10.5°C in thylakoids from cold‐treated and photoactivated leaves, respectively. This indicates that restoration of O 2 evolution as well as of FFA and Mn contents is not accompanied by a complete reversal of membrance conformation.