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Changes in ascorbate, glutathione, and related enzyme activities during thidiazuron‐induced bud break of apple
Author(s) -
Wang Shiow Y.,
Jiao Hong J.,
Faust Miklos
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb00086.x
Subject(s) - dehydroascorbic acid , npsh , ascorbic acid , glutathione , glutathione reductase , chemistry , peroxidase , biochemistry , glutathione peroxidase , enzyme , food science
The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and thidiazuron‐induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non‐protein thiol (NPSH) and non‐glutathione thiol (RSH) occurred as a result of induction by thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.