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Distinct sequence elements in a napin promoter interact in vitro with DNA‐binding proteins from Brassica napus
Author(s) -
Gustavsson HansOlof,
Ellerström Mals,
Stålberg Kjell,
Ezcurra Inés,
Koman Ahmet,
Höglund AnnaStina,
Rask Lars,
Josefsson LarsGöran
Publication year - 1991
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1991.tb00082.x
Subject(s) - brassica , biology , gene , dna , dna binding protein , transcription factor , oligonucleotide , in vitro , dna sequencing , repeated sequence , genetics , transcription (linguistics) , gene expression , electrophoretic mobility shift assay , microbiology and biotechnology , botany , genome , linguistics , philosophy
Nuclear extracts obtained from developing seeds of oilseed rape, Brassica napus cv. Svalöv Karat K 20516, were shown to contain several distinct DNA‐binding proteins as evidenced by gel retardation experiments. Four of the proteins were capable of interacting in vitro with oligonucleotide probes containing sequences related to motives in a napin gene promoter and its upstream region. Another protein interacted with an A/T‐rich repeated sequence present at the 3’end of the gene. The proteins appear to be present also in leaf nuclei and do not show any detectable variations that correlate with napin expression during seed development. Thus, analogous with many transcription factors, the DNA‐binding proteins that we have identified are present in both expressing and non‐expressing cells.