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Histochemical technique: A low‐volume, enzyme‐amplified immunoassay with sub‐fmol sensitivity. Application to measurement of abscisic acid in stomatal guard cells
Author(s) -
Harris Michael J.,
Outlaw William H.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb09068.x
Subject(s) - abscisic acid , guard cell , immunoassay , extant taxon , enzyme , biology , biochemistry , antibody , microbiology and biotechnology , immunology , gene , evolutionary biology
Immunological protocols are useful for quantitative histochemistry because of the high specificity and affinity of antibody‐antigen binding. This interaction, followed by amplification steps, is the basis of an assay that has a sensitivity of 10 ‐10 mol abscisic acid. This assay, combined with extant single‐cell methodologies, makes it possible to determine the cellular localization of abscisic acid within a leaf. This general approach possibly has wide application in plant physiological research; therefore, we give a brief general description of our implementation here.