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Elicitor‐induced hydroxycinnamoyl‐CoA:tyramine hydroxycinnamoyltransferase in plant cell suspension cultures
Author(s) -
Villegas Mirza,
Brodelius Peter E.,
Kylin A.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb09057.x
Subject(s) - elicitor , tyramine , phenylalanine ammonia lyase , catharanthus roseus , biochemistry , phenylalanine , biosynthesis , phaseolus , plant cell , biology , chemistry , enzyme , botany , amino acid , gene
Treatment of Nicotiana glutinosa L. cell suspension cultures with chitosan results in the co‐induction of phenylalanine ammonia lyase, 4‐eoumarate:CoA ligase, tyrosine decarboxylase and tyramine hydroxycinnamoyltransferase, all involved in the biosynthesis of hydroxycinnamoyltyramines. The highest enzyme activities were observed around 12 h after addition of 0.8 to 1 mg chitosan per g fresh weight of cells. No hydroxycinnamoyltyramines could be detected by TLC or HPLC of extracts made from non‐treated or elicited cells. [ 14 C]‐Tyramine was incorporated into insoluble polymeric material at a higher rate in elicitor‐treated than in non‐treated cells of N. glutinosa. Tyramine hydroxycinnamoyltransferase could be induced in suspension cultured cells of Eschscholtzia califortnca Cham, but not in cells of Phaseolus vulgaris L. or Catharanthus roseus (L.) G. Don by addition of a yeast elicitor to the growth medium.