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Effect of alcohols on the association of photosynthetic fructose‐1,6‐bisphosphatase to thylakoid membranes
Author(s) -
Andres A. Rodriguez,
Lazaro J. J.,
Chueca A.,
Hermoso R.,
Gorge J. Lopez
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb09056.x
Subject(s) - thylakoid , fructose 1,6 bisphosphatase , chemistry , membrane , pisum , partition coefficient , ethanol , alcohol , octanol , fructose , biochemistry , methanol , chloroplast , chromatography , organic chemistry , gene
Aliphatic alcohols have a positive effect on the assoociation of pea ( Pisum sativum L. cv. Lincoln) chloroplast fructose‐ 1,6‐bisphosphatase (FBPase; EC 3.1.3.11) with thylakoid membranes. The alcohol concentration needed to obtain a fixed percentage of enzyme association decreased with increased length of the aliphatic chain of the alcohol; maximum binding was obtained when the lysis medium contained, in molar fractions (or v/v percentages): 48×10 ‐4 (T4 (2.4%), 26×10 ‐3 (10%), 40×10 ‐3 (15%), 76×10 ‐3 (21%), and 13×10 ‐2 (24%), of 1‐butanol, 1‐propanol, 2‐propanol, ethanol, and methanol, respectively. A good correlation of binding with the octanol/water partition coefficient was observed. Since this coefficient constitutes a measure of hydrophobicity, we suggest that the binding of FBPase to the membranes occurs via hydrophobic clusters of both components.