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Effects of light on phospholipid metabolism in DunaUella salina
Author(s) -
Peeler Thomas C.,
Thompson Guy A.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb09043.x
Subject(s) - phosphatidylinositol , inositol , phospholipid , metabolism , biochemistry , biology , lipid metabolism , signal transduction , dunaliella salina , phosphatidic acid , chemistry , membrane , botany , receptor , algae
Dunalliella salina (Teodoresco) is a unicellular, wall‐less, halotolerant green alga. Previous work has shown that levels of inositol phospholipiils in whole cells of D. salina fluctuate in response to hyper‐ and hypo‐osmotic shock. In this paper, we report the effects of changes in the light environment on levels of phospholipids, including inositol phospholipids, in D. scilina. Utilizing both short‐term and long‐term labeling of phospholipids with 32 PO 4 , we were able to compare both immediate and long‐term changes in lipid metabolism during changes in the light environment. Relative to the other phospholipids. phosphotidic acid and the inositol phospholipids phosphatidylinositol, phosphatidylinositol 4‐phosphate and phosphatidylinositol 4,5‐bisphosphate were rapidly labeled, even in the dark, suggesting that the metabolism of these compounds is more active than that of the bulk cellular phospholipids. There was little change in inositol phospholipid metabolism when cells were illuminated following a 1 h dark adaptation period, Furthermore, the inositol phospholipid signal transduction pathway did not respond to severe photoinhibition treatment. Apparently this plasma‐membrane‐based signal transduction pathway, which responds to changes in the external environment, is relatively insensitive to major changes in chloroplast metabolism.