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Time‐dependent changes in polypeptide and translatable mRNA levels caused by NaCl in barley roots
Author(s) -
Robinson Nina L.,
Tanaka Chariene K.,
Hurkman William J.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb08726.x
Subject(s) - hordeum vulgare , messenger rna , salt (chemistry) , in vivo , translation (biology) , protein biosynthesis , chemistry , biochemistry , methionine , balanced salt solution , poaceae , biology , botany , amino acid , microbiology and biotechnology , organic chemistry , gene
The effect of salt stress on polypeptide and translatable mRNA levels was examined in roots of barley ( Hordeum vulgare L. cv. CM 72). A salt‐shock time course (200 m M NaCl added to the nutrient solution of 5‐day old seedlings for up to 24 h) and a 6‐day salt treatment (seedlings grown in nutrient solution containing 200 m M NaCl for 6 days) were compared. Roots of intact seedlings were labeled in vivo with [ 35 S]‐methionine. Poly(A) + RNA was isolated and assayed in an in vitro translation system. The changes in the levels of the labeled polypeptides and translation products were analyzed following separation on two‐dimensional polyacrylamide gels. Over the salt‐shock time course, the majority of the changes in polypeptides and translation products were quantitative, as were the changes at 6 days. Qualitative changes occurred during the salt‐shock time course, but were not observed at 6 days. The levels of polypeptides and translation products differed at each point of the salt‐shock time course and at 6 days. In addition, changes were observed at the shortest time points examined, indicating a rapid response to salt.