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Monoterpene glycoside biosynthesis in detached grape berries grown in vitro
Author(s) -
Bravdo B.,
Shoseyov O.,
Ikan R.,
Altman A.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb08720.x
Subject(s) - monoterpene , berry , chemistry , glycoside , callus , botany , thin layer chromatography , chromatography , biology , biochemistry , stereochemistry
A procedure for the culture in vitro of isolated small berries of Vitis vinifera L. cv. Muscat of Alexandria in a Murashige and Skoog basal medium supplemented with N 6 ‐benzyladenine and indoleacetic acid is described. Berries developed well in culture during 60 days and tripled in size, but remained green and smaller than normal berries grown in vivo. Some callus formed on the distal end of the berry, and where major skin damage occurred, callus emerged from the cracked berries. In order to examine their biosynthetic competency, berries which were previously cultured in vitro for 60 days were incubated for 48 h in a Murashige and Skoog medium containing a [ 14 C]‐labelled water‐soluble fraction. This fraction was isolated from grape berries located adjacent to a leaf that had been exposed to gaseous 14 CO 2 in full sunlight for 5 h. The berries were then recultured for 48 h after which a glycosidic fraction was isolated on a C18 reversed phase column and further separated by thin layer chromatography (TLC). The major labelled band corresponded to the geranyl‐β‐rutinoside marker, indicating that grape berries have the ability to synthesize monoterpene glycosides. This band also consisted of other monoterpene glycosides as revealed by the gas chromatography‐mass spectrometry (GC‐MS) analysis of their aglycones (released by enzymatic hydrolysis).