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Inhibition of electron transport activities in mitochondria from avocado and pepper fruit by naturally occurring polyamines
Author(s) -
Phelps Donna C.,
McDonald Roy E.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb08708.x
Subject(s) - spermine , putrescine , spermidine , polyamine oxidase , alternative oxidase , respiration , electron transport chain , polyamine , biochemistry , chemistry , mitochondrion , biology , enzyme , botany
The effects of the naturally occurring polyamines, spermine, putrescine, and spermidine were explored on mitochondrial state 3. state 4, and uncoupled respiration activities, ADP/O ratio, respiratory control ratio of pepper ( Capsicum annuum L. cv. Early Cal Wonder) and avocado ( Persea americana Mill. cv. Booth‐8 or Simmonds) mitochondria oxidizing either succinate, external NADH, malate, α‐ketoglutarate or tetramethyl‐ p ‐phenylenediamine. Abnormally high concentrations of spermine and spermidine such as might occur during chilling stress of these chilling‐sensitive fruits were detrimental to several oxidase activities, especially to external NADH oxidase. State 3 respiration for NADH oxidase was inhibited more than 70% by 10 m M spermine. The spermine inhibition of uncoupled NADH oxidase was not reversed by the presence of divalent cations including Ca 2+ , Mg 2+ , Mn 2+ , and Sr 2+ at concentrations up to 10 m M or by 100 m M KCl. The inhibition primarily affected the V max . Other possible sites of polyamine interactions are discussed.