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Metabolism of tritiated and deuterated gibberellin A 9 in Norway spruce ( Picea abies ) shoots during the period of cone‐bud differentiation
Author(s) -
Moritz Thomas,
Odén Per Christer
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb06737.x
Subject(s) - gibberellin , shoot , picea abies , metabolite , chemistry , gas chromatography , high performance liquid chromatography , chromatography , botany , biochemistry , biology
A mixture of tritiated and deuterated gibberellin A 9 (GA 9 ) was injected into elongating shoots of Norway spruce [ Picea abies (L.) Karst.] grafts grown under environmental conditions that were either inductive (heat and drought, HD) or noninductive (cool and wet, CW) for flowering. The shoots were divided into needles and shoot stems. The metabolites were purified by high performance liquid chromatography (HPLC), detected by liquid scintillation counting of aliquots of collected fractions and identified by gas chromatography‐mass spectrometry (GC‐MS). Deuterated GA 9 was converted to deuterated GA 4 in both treatments. The major metabolite in the CW‐treated material was GA 51 . The HD‐treated material did not convert GA 9 to GA 51 , but a cellulase‐hydrolysable GA 9 ‐conjugate was formed. The same metabolites were found in the shoot stems, though in smaller amounts. The amounts of detected metabolites were higher in the HD material, caused by a higher rate of metabolism and/or smaller losses of the metabolites during sample purification. The estimated amounts of endogenous GAs show that the HD‐treated material contained higher amounts of GA 9 but no differences in the amounts of GA 4 were found.