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New roads towards chloroplast transformation in higher plants
Author(s) -
Haring M. A.,
Block M.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb05891.x
Subject(s) - chloroplast , organelle , nicotiana tabacum , transformation (genetics) , biology , microinjection , homologous recombination , chloroplast dna , chlamydomonas , agrobacterium , dna , genetics , microbiology and biotechnology , gene , botany , mutant
The attempts to manipulate organelle DNA of higher plants have not yet been succesfull. Although some Agrobacterium mediated and direct gene transfer experi‐ments suggested that chloroplasts of Nicotiana tabacum could be transformed, these experiments could not be reproduced. New transformation techniques have emerged: laser‐injection, microinjection and high velocity microprojectiles. The biolistic ex‐periments with Chlamydomonas made it clear that organelle DNA can support introduced DNA fragments by homologous recombination. In view of these devel‐opments, we describe the parameters and new possibilities for chloroplast trans‐formation of higher plants.