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Liposome‐mediated transfer of nucleic acids in plant protoplasts
Author(s) -
Caboche M.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb05882.x
Subject(s) - protoplast , transformation (genetics) , nucleic acid , liposome , dna , biology , kanamycin , plant cell , membrane , gene delivery , microbiology and biotechnology , gene , biophysics , biochemistry , chemistry , genetic enhancement
Liposomes spontaneously interact with the plasma membrane of protoplasts. The delivery of their content to the protoplast, however, has to be induced by physical or chemical treatments. Three major approaches have been used to study and optimize the delivery of RNA or DNA molecules: viral infection, transient gene expression and stable transformation. Conclusive evidence for the delivery of nuclei acids to the treated protoplasts has been obtained. Stable transformants expressing kanamycin resistance have been further characterized by hybridization techniques and progeney analysis. The potential and limitations of this approach for the transformation of higher plants is discussed.