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Cauliflower mosaic virus as a gene expression vector for plants
Author(s) -
Fütterer J.,
Bonneville J. M.,
Hohn T.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb05878.x
Subject(s) - cauliflower mosaic virus , open reading frame , insert (composites) , biology , gene , virus , vector (molecular biology) , mosaic virus , mechanism (biology) , gene expression , expression vector , viral vector , plant virus , genetics , rna , virology , recombinant dna , genetically modified crops , transgene , peptide sequence , mechanical engineering , philosophy , epistemology , engineering
It is possible to replace the CaMV (cauliflower mosaic virus) ORF (open reading frame) II with foreign sequences without interfering with virus viability. Such recom‐binants can induce the synthesis of substantial amounts of a foreign protein in infected plants and confer new properties to these plants. However, so far only three genes have been successfully cloned and expressed in this way. The expression mechanism of CaMV demands precise replacement of ORF II and probably certain structural features of the viral 35S RNA, which should not be disturbed by inserted sequences. Since these features are largely unknown, it cannot at present be pre‐dicted whether an insert will be tolerated. It is more likely that larger inserts will disturb the viral gene expression mechanism than smaller ones.