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Alterations of gene expression in potato ( Solanum commersonii ) during cold acclimation
Author(s) -
Tseng M. J.,
Li P. H.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb05239.x
Subject(s) - acclimatization , hardiness (plants) , biology , messenger rna , rna , botany , population , gene expression , microbiology and biotechnology , gene , horticulture , biochemistry , cultivar , demography , sociology
Plantlets of Solanum commersonii stem‐culture were acclimated at 5°C day/night temperature for 14 days. Cold hardiness increased from – 3.5°C to – 8.6°C. During the course of acclimation, the synthesis of polypeptides was investigated and poly (A + ) RNA was isolated. Translation products of poly(A + ) RNA in a rabbit rcticulocyte lysate system were then analyzed. During the 14 days of acclimation, 23 cold‐induced polypeptides were identified. Most of them disappeared following 1 day of de‐acclimation at a 20/15°C day/night regime. The synthesis of one group of polypeptides is prominent and stable throughout the acclimation period. The other group is transient. The most prominent and stable polypeptides have molecular weights of 21, 22, 31 and 83 kDa. Acclimation alters translatable mRNA population during the development of cold hardiness. Two mRNAs encoding in vitro translation products at 26 and 27 kDa were identified during the course of acclimation. These proteins may play important roles in the overall programming for the development of cold hardiness in tuber‐bearing S. commersonii.

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