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Synthesis of protein and mRNA is necessary for transition of suspension‐cultured Catharanthus roseus cells from the G 1 to the S phase of the cell cycle
Author(s) -
Ohnishi Naoto,
Kodania Hiroaki,
Ando Satoshi,
Komamine Atsushi
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb04380.x
Subject(s) - cycloheximide , catharanthus roseus , protein biosynthesis , dna synthesis , cell cycle , cell division , messenger rna , biology , protein synthesis inhibitor , microbiology and biotechnology , s phase , cell , cell culture , dna , biochemistry , dna replication , gene , genetics , eukaryotic dna replication
The effects of inhibition of the synthesis of protein, mRNA or rRNA on the progression of the cell cycle have been analyzed in cultures of Catharanthus roseus in which cells were induced to divide in synchrony by the double phosphate starvation method. The partial inhibition of protein synthesis at the G 1 phase by anisoniycio or cycloheximide caused the arrest of cells in the G 1 phase or delayed the entry of cells into the S phase. When protein synthesis was partially inhibited at the S phase, cell division occurred to about the same extent as in the control. When asynchronously dividing cells were treated with cycloheximide, cells accumulated in the G 1 phase, as shown by flow‐cytometric analysis. The partial inhibition of mRNA synthesis by α‐amanitin at the G 1 phase caused the arrest of cells in the G 1 phase, although partial inhibition of mRNA synthesis at the S phase had little effect on cell division. In the case of inhibition of synthesis of rRNA by actinomycin D at the G 1 phase, initiation of DNA synthesis was observed, but no subsequent DNA synthesis or the division of cells occurred. However, the addition of actinomycin D during the S phase had no effect on cell division. These results suggest that specific protein(s), required for the progression of the cell cycle, are synthesized in the G 1 phase, and that the mRNA(s) that encode these proteins are also synthesized at the G 1 phase.