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Effect of broad‐band ultraviolet and visible radiation on hydrogen peroxide formation by cultured rose cells
Author(s) -
Murphy Terence M.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb04375.x
Subject(s) - ultraviolet , hydrogen peroxide , peroxidase , radiation , chemistry , photochemistry , biophysics , enzyme , materials science , biochemistry , biology , optics , optoelectronics , physics
Broad‐band radiation from a high‐pressure Hg‐vapor lamp, including ultraviolet wavelengths from 290 to 400 nm, blue, green and red wavelengths, did not induce the synthesis of H 2 O 2 in cultured rose cells. This was in contrast to the effects of shortwave (254 nm) ultraviolet radiation, even though, like shortwave ultraviolet radiation, the UV‐B component of the broadband radiation induced a striking K + efflux from the cells, and this efflux has been associated with H 2 O 2 synthesis in a previous report. The UV‐A and visible wavelengths were shown to inhibit the synthesis of H 2 O 2 . This effect was associated with inhibition of peroxidase, an enzyme reported to be involved in the synthesis of H 2 O 2 in cell walls. UV‐B radiation inhibited the alternate pathway for mitochondrial electron transport, but there was no evidence that this effect contributed to the inhibition of H 2 O 2 synthesis in cells treated with broad‐band radiation.