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3‐Hydroxypropyl amide formation from 1‐aminocyclopropane‐1‐carboxylic acid by a cell‐free ethylene‐forming system from olive leaves
Author(s) -
Vioque Blanca,
FernándezMaculet Juan Carlos
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb02107.x
Subject(s) - ethylene , 1 aminocyclopropane 1 carboxylic acid , olea , chemistry , amide , horseradish peroxidase , yield (engineering) , peroxidase , carboxylic acid , enzyme , biochemistry , organic chemistry , horticulture , biology , catalysis , materials science , metallurgy
The low ethylene yield in a cell‐free ethylene‐forming system from olive tree leaves ( Olea europaea L. cv. Picual) was investigated. During the incubation, 1‐aminocyclopropane‐1‐carboxylic acid (ACC) was extensively transformed into 3‐hydroxypropyl amide (HPA). Enzyme extract, Mn 2+ and oxygen are responsible for this reaction. Horseradish peroxidase (EC 1.11.1.7) can substitute for the enzyme extract in this reaction. HPA formation could be one reason for the poor in vitro conversion efficiency of ACC to ethylene.