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Internode length in Lathyrus odoratus . Effects of mutants l and lb on gibberellin metabolism and levels
Author(s) -
Ross John J.,
Davies Noel W.,
Reid James B.,
Murfet Ian C.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb02102.x
Subject(s) - gibberellin , lathyrus , chemistry , biology , botany , horticulture
After the application of [ 13 C 3 H]‐gibberellin A 20 to wild‐type (tall) sweet peas ( Lathyrus odoratus L.) labelled gibberellin A 1 (GA 1 ), GA 8 , GA 29 and 2‐epiGA 29 were identified as major metabolities by gas chromatography‐mass spectrometry after high performance liquid chromatography. By contrast in genetically comparable dwarf ( II ) plants only labelled GA 29 and 2‐epiGA 29 were produced in significant amounts, although evidence was obtained for trace amounts of labelled GA 1 and GA 8 . The apical portions of dwarf plants contained 8–10 times less GA 1 than those of tall plants but at least as much GA 20 (measured using di‐deuterated internal standards). In conjunction with previous data these results strongly indicate that in genotype ll internode length is reduced and leaf growth altered by a reduction in GA1 levels attributable to a partial block in the 3β‐hydroxylation of GA 20 to GA 1 . In contrast to dwarf plants, semidwarf plants (genotype lblb ) contained more GA 1 in the apical portion than wild‐type counterparts. This is consistent with the suggestion that lb alters some aspect of GA sensitivity.