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Studies on the maintenance and expression of cloned DNA fragments in the nuclear genome of the green alga Chlamydomonas Reinhardtii
Author(s) -
Day Anil,
Debuchy Robert,
Dillewijn Jeanette,
Purton Saul,
Rochaix JeanDavid
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb02089.x
Subject(s) - chlamydomonas reinhardtii , biology , genome , nuclear dna , chlamydomonas , dna , cloning (programming) , gene , nuclear gene , genetics , microbiology and biotechnology , computational biology , mitochondrial dna , computer science , mutant , programming language
Using a biolistic device built here and based on the principle of the device described by Klein et al. (1987). we have reproducibly obtained transformants of Chlamydomonas reinhardtii . The reproducibility of the method has allowed us to examine the maintenance and expression of cloned DNA fragments introduced into C. Reinhardtii .