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Storage protein accumulation during zygotic and somatic embryo development in Picea abies (Norway spruce)
Author(s) -
Hakman Inger,
Stabel Priska,
Engström Peter,
Eriksson Tage
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb00065.x
Subject(s) - picea abies , somatic embryogenesis , embryo , biology , storage protein , somatic cell , zygote , gel electrophoresis , isoelectric focusing , polyacrylamide gel electrophoresis , botany , biochemistry , embryogenesis , microbiology and biotechnology , gene , enzyme
Total protein was extracted from zygotic embryos and from somatic embryos of Picea abies (L.) Karst. (Norway spruce) cultured in vitro at different times during their development. An analysis by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and 2‐dimensional gel electrophoresis of the protein extracts showed that protein composition and the temporal changes in protein abundance were very similar in the two embryo types. Both zygotic and somatic embryos accumulated storage proteins in abundance during their maturation phase of growth; the somatic embryos when cultured on medium containing 90 m M sucrose and 7.6 μ M ABA. The major storage proteins are composed of polypeptides with molecular masses of about 22, 28, 33 and 42 kDa and they are identical in both embryo types according to their molecular mass and average isoelectric points. These proteins are also the most abundant proteins in the female gametophytic tissue of the mature seed.