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Slow‐phase chlorophyll fluorescence characteristics of the donor plant leaf tissue as a predictive parameter of protoplast survival potential in vitro
Author(s) -
Cassells Alan C.,
Hurley Deirdre H.
Publication year - 1990
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1990.tb00064.x
Subject(s) - protoplast , nicotiana tabacum , biology , chlorophyll fluorescence , in vitro , solanaceae , chlorophyll , botany , horticulture , biochemistry , gene
Variation in the steady state (F T ) to fluorescence peak (F P ) ratio of Nicotiana tabacum L. cv. Xanthi‐nc leaves in relation to their position on the plant were correlated with the survival in vitro of protoplasts isolated. The F T /F P ratio detected variation in the protoplast survival potential in individual plants in a batch and between batches of donor plants. Deliberately waterlogged and droughted plants had increased F T /F P values and decreased protoplast survival potential. The use of slow‐phase chlorophyll fluorescence characteristics as a predictive parameter of protoplast survival potential is discussed and compared with the use of stress ethylene and stress ethane measurements for the same purpose.