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Fiber development in preanthesis cotton ovules
Author(s) -
Davidonis Gayle H.
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb06183.x
Subject(s) - ovule , kinetin , callus , botany , biology , tissue culture , in vitro , murashige and skoog medium , horticulture , biochemistry , pollen
A tissue culture method was developed to investigate the production of cotton (Gossypium hirsutum L. cv. Texas Marker‐1) fibers in vitro. Ovules were excised from 3, 5, 7 and 9 days preanthesis ovaries and placed on an agar‐solidified, modified Murashige and Skoog medium containing 2.3 μ M kinetin and 0.45 μ M –2,4–dichlo‐rophenoxyacetic acid or 2.3 μ M kinetin and 10.7 μ M naphthaleneacetic acid. Ovules formed fibers and callus tissue. Fibers formed in vitro were up to 10 mm long, 10–22 μ wide and the cell wall was 1–3 μ M thick. Callus tissue cells were subcultured for over 25 weeks and their degree of elongation was monitored. The ability of ovule‐derived cells to direct expansion in a longitudinal direction diminished, while lateral expansion increased with time in culture.