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Regulation of abscisic acid translocation during embryo maturation of Phaseolus vulgaris
Author(s) -
PageDegivry Marie Thérèse,
Barthe Philippe,
Prévost Isabelle,
Boulon Brigitte
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05981.x
Subject(s) - abscisic acid , phaseolus , embryo , endogeny , point of delivery , biology , chromosomal translocation , microbiology and biotechnology , embryogenesis , botany , chemistry , biochemistry , gene
When R, S [2‐ 14 C] abscisic acid (ABA) was applied to the leaf of Phaseolus vulgaris , a part of the radioactivity was always found 24 h later in the only pod left on the plant. In early podfill, a large part of the labeled material was found in the maternal tissues while in late podfill, most had migrated to the embryos. During embryogenesis, embryo cells became more and more alkaline with respect to the seed coat cells. These results suggest that the distribution of ABA within the tissue is regulated by the pH differential between the two compartments. The decrease of endogenous ABA level observed in situ during the second part of the embryo development therefore cannot be explained only in terms of the passive diffusion of the undissociated species (ABA‐H). The empty‐ovules technique revealed that ABA was only partly affected by an inversion of the pH gradient and that metabolic inhibitors influenced the release of ABA. These results indicate that in addition to a diffusive path, an energy‐dependent component was involved.