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Metabolism of tritiated and deuterated gibberellins A 1 , A 4 and A 9 in Sitka spruce ( Picea sitchensis ) shoots during the period of cone‐bud differentiation
Author(s) -
Moritz Thomas,
Philipson J. Julian,
Odén Per Christer
Publication year - 1989
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/j.1399-3054.1989.tb05975.x
Subject(s) - deuterium , gibberellin , chemistry , chromatography , metabolite , shoot , high performance liquid chromatography , mass spectrometry , gas chromatography , radiochemistry , botany , biochemistry , physics , quantum mechanics , biology
A mixture of tritiated and deuterated gibberellins (GAs) was injected into elongating shoots of Sitka spruce [ Picea sitchensis (Bong.) Carr.] grafts grown under environmental conditions that were either inductive (heat and drought, HD) or non‐inductive (cool and wet, CW) for flowering. The metabolites were purified by high performance liquid chromatography (HPLC), detected by liquid scintillation counting of aliquots of collected fractions and identified by gas chromatography–mass spectrometry (GC‐MS). Deuterated GA 9 was converted to deuterated GA 4 , deuterated GA 34 , and deuterated GA 1 in both treatments. Deuterated GA 4 was metabolized to deuterated GA 34 and deuterated GA 1 in the CW material, but only deuterated GA 1 was detected in the HD material. The amount of detected metabolites was higher in the HD material, caused by a higher rate of metabolism and/or smaller losses of the metabolites during sample purification. GA 1 was converted to a polar unidentified metabolite in both treatments, but to a higher degree in the CW treatment.